THE UNIVERSITY OF CRIMINAL INVESTIGATION AND POLICE STUDIES

DOCTORAL STUDIES

Forensic Engineering

Course:

^{Chromatography Methods}

Course Code: IF4

ECTS: 15

Course Status:

Elective

Number of Effective Classes (Per Week): 5

Theoretical Education:

Practical Training:

Research Study Project:

5

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Prerequisite/s: None

Educational Objective: Acquiring knowledge on the use of chromatography methods in the identification of chemical and biological materials.

Projected Outcome: Learning about gas and liquid chromatography, basic principles, LC, HPLC methods, combined GLC – mass chromatography, MALDI. Knowledge required for the preparation of samples for chromatography, solvent choice, detection techniques. Familiarity with the basic principles of interpreting measured spectra.

Course Contents/Structure

Theoretical education: Theoretical foundations of mass spectrometry applied on gas and liquid samples. Basic processes of molecule transportation in gases and liquids and interaction with surfaces. Techniques of sample preparation: aerosols, particles and volatile molecules. Laser evaporation, MALDI, cathode spraying. Choice of solvents, chemical properties and compatibility. Getting acquainted with the principles underlying the operation of diagnostic devices generally and the signal detection section in particular. Basic principles of interpreting the obtained data.

Teaching Methods: Mentorship, lectures, interactive instruction, seminars, laboratory work.

Assessment (Maximum Number of Points: 100)

Pre-Exam Obligations

No. of Points

Final Examination

No. of Points

Study paper

30

Exam

70

Textbook/s

1. Scott, R. P. W. Principles and Practice of Chromatography, Lancashire: Reese – Scott Partnership, 2012.

2. Snyder, L. R. Introduction to modern liquid chromatography (3^rd ed.), Hoboken: Wiley, 2009.